DETECTION OF GENETIC VARIABILITY AMONG SUGARCANE SOMACLONES USING SSR MARKER

Abstract

The experiment was conducted for detection of genetic variability among ten sugarcane somaclones including TC2-10(16), TC3-10(16), TC5-10(16), TC8-10(16), TC10-10(16), TC1- 10(20) PEG, TC3-10(20) PEG, TC7-10(20) PEG, TC16-10(20) PEG and TC23-10(20) PEG along with their parent varieties (Isd 16 and Isd 20) using two Microsatellite (SSR) markers UGSM 565 and UGSM 567. The sizes of amplified bands in ten sugarcane somaclones of two varieties Isd 16 and Isd 20 ranged from 80 to 650bp. Two SSR markers amplified a total of 58 bands. SSR markers UGSM 565 and UGSM 567 amplified 25 and 33 number of bands, respectively. The highest number of bands (2.75) per variety was amplified from UGSM 567 marker and lowest number of bands (2.08) per variety were recorded from UGSM 565 marker. Genetic diversity or polymorphic information content (PIC) for UGSM 565 was 0.64 and for UGSM 567 it was 0.69 with a mean of 0.67 for all loci across ten somaclones and their parent varieties evaluated. The highest linkage distance 6.0 and lowest linkage distance 1.0 were recorded among ten somaclones and two parent varieties. Genetic relationships based on UGSM 565 among ten somaclones along with their two parent varieties at the average distance of 4.95 showed two major clusters. The somaclones TC23-10(20) PEG and TC10-10(16) were separated from other investigated somaclones and varieties. On the other hand, based on UGSM 567 genetic relationships among ten somaclones along with their two parent varieties at the average distance of 5.5 showed two major clusters and somaclones TC23-10(20) PEG and TC10-10(16) were separated from other somaclones and their parent varieries respectively. The results revealed that two SSR markers were able to detect/identify and classify ten sugarcane somaclones along with their parent varieties indicating genetic differences among somaclones and their parent varieties.