EFFECT OF MEDIUM COMPOSITION ON CALLOGENESIS AND SOMATIC EMBRYOGENESIS IN DIFFERENT VARIETIES OF SUGARCANE (SACCHRUM OFFICINARUM L.) I.E. S-2002-US-302, HSF-240 AND HSF-242

Abstract

Segments from inner young leaf whorls (of 3-9mm length) of sugarcane were cultured on MS growth medium supplemented with auxin alone i-e. 2,4-D (1-4 mg/l) and auxin-cytokinin combinations in different ratios. Leaf tissue explants had great potential for callus induction, somatic embryoid induction and supported plant regeneration. After inoculation embryogenic callus (of varied texture and color) was formed while in some cases direct embryoid emergence on cut edges of explant was also exhibited. Among MS media with auxin concentrations 2,4-D 3mg/l was more appropriate for callus and somatic embryoid induction as well as plant regeneration in all three varieties. Each variety showed different response to different ratios of auxin-cytokinin combinations.2,4-D+BAP (2+1, 2+2 & 2+3mg/l) and IAA+BAP (2+2 & 2+3mg/l) were more appropriate for callus and embryo induction and plant regeneration. Abbreviations: BAP: 6-Benzyl aminopurine, 2,4-D: 2,4-Dichlorophenoxyacetic acid, IAA: Indole acetic acid, MS: Murashige and Skoog‟s growth medium, EDTA: Ethyline diamine tetra acetate.